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The next day, cells were strained through a 35-m nylon mesh and sorted on a BD FACSAria Fusion (BD Biosciences) operated on BD FACSDiva software (version 8.0.2) using a 70-m nozzle excluding aggregate, dead (Zombie NIR-positive) and reporter-negative (BFP-negative and mCherry-negative) cells

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Some independent researchers recommend taking the full daily dose on an empty stomach, first thing in the morning

PubMed Diamond, L.E., Earle, D.C., Rosen, R.C., Willett, M.S., & Molinoff, P.B