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Briefly, frozen-thawed spermatozoa from each group (repeated in triplicated) were collected, centrifuged at 500 g for 10 min, and diluted with phosphate buffer saline (PBS) solution

Subsequent to this, a brief equilibration phase of 3,000 steps was initiated, during which temperature and pressure were gradually adjusted to 300 K and 1 atm, respectively

As FAO feeds OXPHOS 51 and produces ROS 52 , it is rational to observe these changes together

Ab0475 Transcranial Direct Current Stimulation Decreases Chronic Pain in Patients with Rheumatoid Arthritis: A Randomized, Controlled, Double-Blind Clinical Trial