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Neutrophils were then stimulated with PVL (150 nmol/L) at room temperature for 45 min, or with 100 nmol/L PMA for 2 h at 37 C, 5%CO 2 , followed by addition of Sytox green (1 mol/L) and Hoechst33342 (5 g/mL) for a further 15 min in the dark

While natural glutathione is primarily produced by our cells on their own, unfortunately, glutathione levels in the body are often reduced by a number of factors and decline over the years