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At regular time intervals, 2 mL of the supernatant was removed for UV-vis spectrophotometry at = 292 nm and 380 nm corresponding to LEV and QS, respectively, and the solution was replenished

Additionally, ROS-induced oxidative modifications of cellular macromolecules generate damage-associated molecular patterns (DAMPs), which are recognized by pattern recognition receptors, such as Toll-like receptors (TLRs), thereby perpetuating sterile inflammatory responses (Koenig and Buskiewicz-Koenig, 2022)

Role of hydroperoxyeicosatetraenoic acids in oxidative stress-induced activating protein 1 (AP-1) activity

Chandra V, Sharma GT