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For the western blotting of HMGB1, frozen cortices were homogenized in 50 mM TrisHCl (pH 7.5) containing protease inhibitor cocktail and phosphatase inhibitor cocktail

sTFR was also found to be significantly and positively correlated with parameters of inflammatory activity and autoimmune disease ( Iron, as a regulator of the immune response, affects the phagocytosis of monocytes and macrophages