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The qPCR was performed using PerfeCTa SYBR Green SuperMix (Quantabio) conducted on an ABI StepOne Real-Time System following the cycles: 95 C for 3 min and 40 cycles of 95 C for 10 s and 60 C for 30 s

Estrogen tends to moderate stimulation of the heart and provides cardioprotective benefits in general

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