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Ethics statement The animal study was approved by Ethics Committee of Laboratory Animal Welfare, Affiliated Hospital of Qingdao University

Also, pericytes in the BBB differ from pericytes in the body in their numbers, that is, there is a large number of pericytes located in the CNS compared to other body tissues, thereby making the ratio of pericytes to endothelial cells in the CNS higher (1:1 to 1:3) compared to other cells, such as the muscles (1:100)

Kilic M, Ozgl RK, Coskun T, et al

Then, they underwent routine histopathological processing, embedding in paraffin, sectioning to 5 m thickness, and staining with hematoxylin and eosin