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At 24 h post transfection, cells were washed with PBS before addition of 200 l fresh GMEM including 0.5 M of Alexa 647 labelled Nb1_gbHSV nanobody

Aptamers as versatile ligands for biomedical and pharmaceutical applications

The circadian pacemaker, located in the suprachiasmatic nuclei (SCN) of the hypothalamus, interacts with the sleep homeostatic system to regulate consolidated periods of wakefulness and sleep aligned with the daynight cycle 6