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doi: 10.1016/S0960-9822(02)00809-6 229 HiguchiFUchidaSYamagataHAbe-HiguchiNHobaraTHaraKet al

NAM relieves mitochondrial ROS by enhancing the expression or activity mitochondrial antioxidant enzymes To determine the effect of NAM on the cellular oxidative stress level, MC3T3-E1 cells were stained with a fluorogenic probe, CellROX TM , which measures the ROS level in the cytosol, and MitoSOX TM , which measures superoxide production in mitochondria