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Collectively, these findings suggest that the M5 strategy supports stable early subculture expansion and confers a proliferative advantage, particularly in terms of reduced PDT at later early passages

In conclusion, the gut microbiota-hepatic ferroptosis plays a crucial role in the pathogenesis of ALD, targeting the modulation of gut microbiota while inhibiting ferroptosis presents a promising therapeutic intervention to mitigate liver injury and improve outcomes for individuals affected by ALD

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