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The so-called gatekeeper residue, which usually features a conserved bulky phenylalanine in plant PPOs, is located inside the pocket of the active center and shields CuA 27

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With the homozygous pGSH1-URA3 strain, a ~ 50% increase of GSH cellular content was observed in cultures supplemented with 100 g/ml 5-FOA, and this increase was relatively stable compared to that of the previous heterozygous strain (Figure S4A)

Combined Expression of Tau and the Harlequin Mouse Mutation Leads to Increased Mitochondrial Dysfunction, Tau Pathology and Neurodegeneration