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Cell Metab (2006) 4:4212

CPT: Pharmacometrics & Systems Pharmacology 1(9):e2 Rasmussen BB, Brix TH, Kyvik KO, Brosen K (2002) The interindividual differences in the 3-demthylation of caffeine alias CYP1A2 is determined by both genetic and environmental factors

The resulting mixture is electroporated (1600 pulse voltage, 10 millisecond pulse width, 3 pulses) with 200,000 cells in electroporation buffer and the cells are deposited into antibiotic free media (see Cell Lines and Culture for conditions) and allowed to rest in a 37 C incubator for 24 h before additional antibiotic containing media is added