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The non- enzymatic antioxidants may protect the spermatozoa from oxidative DNA and membrane disruption by decreasing the singlet oxygen and the detrimental effect of lipid peroxidation on sperm [65]

In this probe the BODIPY fluorophore was conjugated via a dienyl group to a phenyl group, and, similarly to MitoSOX, the specificity for the inner membrane of the mitochondria (facing the matrix) is given by a triphenylphosphonium lipophilic cation, whose uptake is driven by the mitochondrial membrane potential