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Proteoliposome counterflow assays Frozen proteoliposome and protein-free liposome aliquots (lipid concentration: 8 mg/ml) were thawed and diluted twofold in internal liposome buffer with final concentrations of 150mM KCl, 50mM HEPES (pH 7.4), 5 mM (Figure 3a and Extended Data Figure 4l) or 10 mM (Extended Data Figure 7F) unlabeled GSSG (Thermo J63715.06)

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Biochemistry 39:1300513011 Vitvitsky V, Dayal S, Stabler S, Zhou Y, Wang H et al (2004) Perturbations in homocysteine-linked redox homeostasis in a murine model for hyperhomocysteinemia

SIRT2 Mediates NADH-Induced Increases in Nrf2, GCL, and Glutathione by Modulating Akt Phosphorylation in PC12 Cells