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Conflicts of Interest The authors declare no conflict of interest

Daily NNMT inhibitor effects on body weight measures in DIO mice was analyzed using a repeated measures two-way ANOVA with Sidaks multiple comparison posthoc test

Methylation Capacity Second, NNMT inhibition affects cellular methylation capacity: SAM is required for hundreds of methyltransferase reactions throughout the cell, and NNMT's consumption of SAM can deplete this critical resource, while the SAH produced by NNMT is a potent product inhibitor of many methyltransferases

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