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Purity of the protein preparation was estimated by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) based on the method described by Laemmli [60] using a 12% precast polyacrylamide gel (Bio-Rad, USA)

Therefore, when its levels are high, as in cardiac muscle cells after epinephrine stimulation or in skeletal muscle cells during muscular contraction, the phosphatase is active, the complex is dephosphorylated, and thus active

Defining where this chemistry occurs in the cell can inform the design of drugs capable of inducing or inhibiting ferroptosis in various disease-relevant settings

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