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doi:10.1038/emm.2007.88 Rothstein JD, Dykes-Hoberg M, Pardo CA, Bristol LA, Jin L, Kuncl RW, Kanai Y, Hediger MA et al (1996) Knockout of glutamate transporters reveals a major role for astroglial transport in excitotoxicity and clearance of glutamate

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Intracellular ferrous iron (Fe) was measured with an enzyme-linked immunosorbent assay (ELISA)-based kit (Wuhan Elabscience Biotechnology Co., Ltd., China), following cell collection, washing, lysis, and processing as per the manufacturers instructions, with absorbance read at 450 nm