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Neuronal loss and decreased GLT-1 expression observed in the spinal cord of Pembroke Welsh Corgi dogs with canine degenerative myelopathy

Tissue sections or fixed endothelial cells were processed for immunolabeling by permeabilization (0.4% Triton X-100 in PBS), blocking (4% horse serum, 2% BSA, 0.2% Triton X-100 in PBS) and incubation with first antibody (1% HS, 0.05% Triton X-100 in PBS)

This process is similar to the mobilization and utilization of axunge by the body during exercise or starvation, transforming axunge from a stored state to a state available for energy use

Nanosci Master Progr 109:120 Toh SY, Citartan M, Gopinath SCB, Tang T-H (2015) Aptamers as a replacement for antibodies in enzyme-linked immunosorbent assay