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After washing, cells were incubated with secondary antibody, Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H + L) (711-585-152, Jackson ImmunoResearch Laboratories, West Grove, Pennsylvania, USA), for 1 h at RT

At the end of experiments, the rats were anaesthetised by isoflurane and euthanised by exsanguination

In line with this, a recent study aiming at identifying genes, mutations and biological processes that give selective advantage to mutant clones in blood cancer, identified FH to harbour copy-neutral loss of heterozygosity

Oxidative stress in diabetic peripheral neuropathy: pathway and mechanism-based treatment