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Trichloroacetic acid was added to homogenise the tissue samples, which were then centrifuged (3000 rpm, 5 min), and the supernatant was collected

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Although studies suggested that tryptophan and its derivatives could influence the gut microbiome and interact with the gut mucosal immune system, recent clinical investigations proposed that changes in immunity and kynurenine metabolism may trigger fatigue in ME/CFS but do not sustain it over time [61, 67, 68]

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