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Excessive mitochondrial production of ROS has been implicated in the pathogenesis of HF and AF [31]

To assess mitochondrial morphology parasites were grown in an HFF monolayer on a glass coverslip in the presence or absence of ATc for three days and an immunofluorescence assay performed using primary antibodies against the mitochondrial protein TOM40 (1:1000, anti-rabbit, (34))

Disrupted proteostasis and autophagy-lysosomal dysfunction The autophagy-lysosomal pathway (ALP) and ubiquitin-proteasome system (UPS) are critical for degrading misfolded proteins