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The two supernatants were combined, the pH was adjusted to 5 with potassium hydroxide, a 10 L aliquot was collected for LC-MS analysis and the sample was frozen-dried

Ascorbic acid was shown to cause Dev R regulation by an extra process

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This process promotes the sequestration of calcium ions in the sarcoplasmic reticulum while reducing their concentration in the cytosol, which impacts phosphorylation and consequently results in smooth muscle relaxation (Fig