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Sections were immersed in a buffer containing terminal deoxynucleotidyl transferase and digoxigenin-labelled nucleotides for 2 h at 37 C

In an effort to clarify the role of the KEAP1-NRF2 pathway in ALS, it has been revealed that the transfection of the mouse MN-like hybrid cell line (NSC-34) with the human SOD1 G93A gene causes damage in the Nrf2/ARE signalling and a reduced ability of cells to react to OS

Scientifically, multidimensional classification frameworks are evolving to integrate NCCD genetic criteria, metabolic signatures (e.g., intracellular GSH/GSSG ratios), and microenvironmental features defined by spatial transcriptomics, enabling prediction of dominant death pathways