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During the procedure the animal is placed on a heating pad (40 C.) in a sound proof booth and an earphone is fitted loosely into one ear at a time

BMPs regulate NSC differentiation into astrocytes by acting through the phosphorylation of Smad proteins 1, 5, and 8, which combine with Smad4 in order to form a complex that translocates to the nucleus and regulates gene transcription (Itoh et al., 2000