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After 4 h of incubation at 37 C, the medium was replaced with 100 l DMSO, and absorbance was measured at 540 nm using a microplate reader (Elx800, BioTek, USA)

Tony Urey about the idea that amyloidosis can be a multisystem diseasewhile clinical attention has often centered on cardiac manifestations, particularly in wild-type transthyretin amyloidosis, which has historically been framed primarily as a cardiac condition

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