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S.RoyS.MittlerR.et al (2019)

Simplistic conventional, also inappropriately called 2D, monolayer cultures with a single layer of cells attached to treated polystyrene have unnaturally low cell densities, lack cell-cell interaction or cell-peri and extracellular matrix interactions, and lack the exhibition of the brain tumor nutrient gradients or physiological levels of oxygen (112)

Its main mechanism appears to be its interaction with the nitric oxide (NO) system and its ability to upregulate growth factors like Vascular Endothelial Growth Factor (VEGF)

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