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Samples were loaded into 5 L standard-treated capillaries (Hirschmann, Germany) after the reactions were incubated in the dark at 25 C for 30 min to 1 h

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In order to investigate whether it is attributed to the inability of VP35 to bind to heterologous L protein or the chimeric complex is inactive, we conducted co-expression and pull-down experiments using different combinations of LVP35 pairs