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Cell viability was determined by mixing an aliquot of the suspension 1:2 with trypan blue and counting viable cells using a hemocytometer

L-PAM as a single agent was highly active against MM.1S, KMS-12-PE, MOLP-2 and NCI-H929, inducing 2 logs of cell kills at the maximum dose (50 M)

Many immunomodulatory factors are found in the tegument, the multi-layered outer surface of the parasite which is capable of quick self-renewal after damage, a process mediated by a population of somatic stem cells with a propensity to differentiate into tegument associated cells (147).The factors within the tegument have been extensively examined (148, 149)

After permeabilization and blocking, primary antibody was added and incubated for 1 h at room temperature