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Under these conditions, it may help to add a stretch of glycines, or histidine or a flag tag between the TEV cleavage site and the N-terminus of the target protein

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As finalized in the FY 2021 IPPS/LTCH PPS final rule (85 FR 58888), we proposed that subsection (d) hospitals in the 50 states and DC, as defined at section 1886(d)(1)(B) of the Act, and subsection (d) Puerto Rico hospitals, as defined under section 1886(d)(9)(A) of the Act, would be required to report the median payer-specific negotiated charge information