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Studies of another thiol-containing antioxidant N -acetylcysteine (NAC), at its intrinsic pH, showed that it destabilizes the biofilm matrix by chelating divalent cations (e.g., Ca 2+ and Mg 2+ ), inhibition of bacterial extracellular polysaccharide production, modulation of bacterial cell physiology and the impact on its metabolism (Zhao and Liu, 2010

J.et al (2018)

The affinity is different in this process, with Cu showing a higher affinity compared to Fe or Zn

J Japan Soc Food Sci Technol-Nippon Shokuhin Kagaku Kogaku Kaishi 53:3138 Lin F, Wang R (2010) Hemolytic mechanism of dioscin proposed by molecular dynamics simulations