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36 Conversely, BMAL1 knockout in chondrocytes ( Col21-CreER, Bmal1 flox/flox ) significantly reduces VEGF and HIF-1 levels while increasing MMP13 and Runx2 expression, ultimately suppressing cell proliferation and promoting apoptosis

The raw RNA sequencing data was mapped to human reference genome hg38 using the STAR aligner 63 , and genes annotated in Gencode v36 64 was quantified using featurecounts in the subread package 65

1 probiotic formula in two animal models of colitis

Locally produced IGF-1 also contributes to autocrine and paracrine signallingtissues produce their own IGF-1 in response to GH stimulation, creating local high concentrations even when systemic IGF-1 remains modest