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For example, the general applications of adeno-associated virus-based gene therapy are hampered by pre-existing humoral immune responses against the viral vectors [350,351,352], which for some viral serotypes occur in up to 60% of the population because of early-life infections [353, 354]

The main findings are: (a) a change in the crustal composition in Nicaragua is characterized by an increase in regional gravity values from the Pacific Coastal Plain to the Atlantic Coastal Plain, (b) the boundary between the CHOB and the DPM are by a series of northeast-southwest oriented residual anomaly lineaments associated with the Jalan and Guayape faults, c) the horizontal extension of the DPM is inferred in the northern Nicaragua over an area of ~ 37,500 km 2 , d) the MCOT extends northward into Costa Rica territory adjacent to the CLIP, where regional gravity anomaly values increase to 75