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The suspension was transferred to 36 mL ultracentrifuge tubes (Beckman Coulter, 355618) and centrifuged at 300,000x g (max) at 4 C for 60 min in a T-865 fixed angle rotor (Thermo Scientific, 51411) and Sorvall WX + Ultra centrifuge (Thermo Fisher, 75000100)

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Approximately 0.81.2% of children who are referred to a pediatric endocrinologist for suspected slow statural growth are diagnosed with SPIGFD, based on a French cohort study [2]