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doi:10.1016/j.socscimed.2017.12.005

In Neurospora crassa , molecular analyses delineated distinct anabolic functions for NADP-GDH and catabolic functions for NAD-GDH, with the am gene encoding NADP-GDH extensively characterized at biochemical and genetic levels [54, 55]

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The beads were collected and washed, and the bound proteins were eluted by Laemmli buffer containing 500 L 6 M urea, 25 L 200 mM DTT, and 25 L 500 mM IAA in the dark at 25C for 30 minutes