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After a further 24 h, the cells were washed with cold PBS, lysed by sonication (4 C, 5 min), and centrifuged at 10,000 g for 10 min

These enzymes eliminate the effects of superoxide radicals and protect the cells against the harmful effects of these radicals

Beginning in 1963, the Schoenenberger-Monnier research group investigated whether specific compounds circulating in the bloodstream might contribute to the initiation and maintenance of sleep