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Finally, the purified DNA samples were subjected to reverse transcription-quantitative polymerase chain reaction analysis and high-throughput sequencing

doi: 10.1016/j.pupt.2014.05.002 141 MobarraN.ShanakiM.EhteramH.NasiriH.SahmaniM.SaeidiM.et al

ECM, energy corrected milk

To determine cell viability, 3,000 HT-1080 or MDA-MB-231 cells were seeded in a 96-well plate with standard medium (10% FBS and 1% penicillinstreptomycin) around 20 h before treatment