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Glutathione exists in two forms: reduced (GSH) and oxidized (GSSG)

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For total NAC in aqueous humor, the same procedure was used, substituting aqueous humor for plasma, with the following modifications: 4.0 L aliquot of ammonium hydroxide, 25 L aliquot of 200 ng/mL NAC-d3 in 0.5 mg/mL disodium EDTA (pH 7.0), 10.0 L of 100 mM ammonium carbonate, 5.0 L of CMPI (60 mM), 5.0 L of TCEP (60 mM), 300 L of acetonitrile, and 150 L of supernatant