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The effect of CYP on the isolated tissue was tested using the following parameters: rate of spontaneous contractions and cystometric parameters including intercontraction interval

In another set of experiments, ASA treatments (5 or 10 mol/ml ASA 48 h) were performed in GSH-depleted cells (BSO treatment for 18 h) which were then treated with 10 mM NAC (dissolved in DMSO, then diluted with cell culture medium to the required concentration keeping the DMSO concentration not more than 0.1%) for 2 hr before the addition of ASA for 48 h to manipulate cellular GSH pools

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