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Description

GST-NMT1 was eluted in GST-Elution Buffer (50 mM Tris/HCl pH 8.0, 100 mM NaCl, 5% glycerol, 20 mM reduced glutathione, 1 mM DTT) for 1 h at 4 C

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Proteomes for bioinformatic prediction of serine hydrolases were downloaded from UniProt, and accession codes for each proteome are included in Supplementary Table 2