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HeLa cells were treated with a series of concentrations of buthionine sulfoximine (BSO) for 72 h, a specific inhibitor for the enzyme required in the first step of GSH synthesis (Fig

Both of these effects contribute to the impressive stability of the GSH concentration, seen in Panel B, in the face of large fluctuations in amino acid input

Owing to its high concentration in the cell (~10 3 M) 1 , the GSH/GSSG pair acts as the main intracellular redox buffer, plays a key role in the detoxification of reactive oxygen species (ROS), and participates in redox signalling 2

Mosaic Diagnostics uses a method for creatinine correction on each individual sample