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Then, 4 l of reagent B and 20 l of trichloroacetic acid were simultaneously added

Alterations in gut microbiota can disrupt neurotransmitter homeostasis, particularly affecting serotonin (5-HT), norepinephrine (NE), and dopamine (DA) systems that are dysregulated in PTSD (Zhou et al., 2020)

Western immunoblotting was conducted to examine protein expression related to key signaling pathways and cellular stress responses