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Finally, the tissue sections were washed thoroughly with PBS, counterstained with DAPI and imaged under a laser scanning confocal microscope (FV-1000, Olympus, Japan)

Xenotransplantation-based models and drug treatment NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL-3,CSF2,KITLG)1Eav/MloySzJ mice expressing human IL-3, GM-CSF and SCF on a NSG background (NSG-S mice) were purchased from Jackson Laboratory (Bar Harbor, ME, USA)

In aquafeeds, glutamine is typically supplemented as L-glutamine, the bioavailable and stable free form used to meet increased demands under stress