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10.1016/j.arr.2007.08.007 30

The pooled sample was dephosphorylated by first adding 80 l SAP buffer (200 mM Hepes pH 7.8, 100 mM MgCl2) and 2 l Shrimp Alkaline Phosphatase (USB, 40 U/l) to the pooled sample followed by incubation of the sample at 37C for 1 hour