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After neutralization with 0.5 M phosphate buffer (7.5), 0.1 mL of the supernatant was added to a reaction mixture containing 0.2 mM NADPH, 100 mM phosphate buffer (pH 7.5), 5 mM EDTA, and 0.6 mM 5,5dithio-bis (2-nitrobenzoic acid)

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Potential subjects remaining eligible after initial screening underwent MRI to assess liver fat content eligibility of 10% by proton density fat fraction (PDFF) and fibro-inflammation defined as corrected T1 (cT1) 800 msec (step 2)

In the metazoan model Caenorhabditis elegans , the uncharacterized gene H24K24.3 codes for the ortholog of the human ADH5 enzyme