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A general strategy for the evolution of bond-forming enzymes using yeast display

To interpret the binding isotope effects quantitatively, we performed ab initio calculations that were then used to calculate binding isotope effects.[75, 81] The C-H bond of a model 5-deoxypyridoxal-Ala imine was incrementally lengthened and held fixed while the geometry of the remaining atoms was optimized

An interesting study investigating substrate oxidative metabolism in brain cellular models showed that oxidation of KBs by neurons and oligodendrocytes is three times more efficient than that by astrocytes (Edmond et al., 1987)

doi: 10.1016/j.jtemb.2018.02.024 113 StoneJR