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By systematically reviewing and synthesizing existing literature, this article aims to provide insights for further investigation into ferroptosis mechanisms in OA and to broaden potential therapeutic targets and approaches for clinical management of ferroptosis-related pathological processes

Live intracellular fluorescence stainings CellROX Orange Reagent, for oxidative stress detection (C10443, Invitrogen, Thermo Fisher Scientific), FerroOrange (F374, Dojindo), and BODIPY 581/591 C11 (D3861, Invitrogen, Thermo Fisher Scientific) were used according to the manufacturers instructions

Firstly, it uses about 20% of the bodys oxygen supply for energy production (and energy production is a significant source of reactive oxygen species (ROS)

Except for S8 site, most of the hotspots did not coincide with experimental fragment binding sites