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The researchers added Epitalon to the culture medium and assessed oocyte quality across 6, 12, and 24 hours of post-ovulatory aging

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Glutathione appeared to act as a catalyst that accelerated zoospore release by at least 12 h compared to the control, and this increased zoospore production was sustained during the growth cycle (Figure 2)

Supernatants were applied to a HisTrap FF column and eluted with a linear gradient of imidazole, then further purified using a Superdex 200 column