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2.5 Liver tissue oxidant/antioxidant activity evaluation Following the protocol laid out earlier, the liver homogenate was produced (Paglia and Valentine, 1967

doi: 10.1016/j.ijbiomac.2025.149828 66

Research has demonstrated that the accumulation and deposition of MSU crystals in the joint cavity can enhance oxidative stress, releasing large quantities of oxidants such as ROS, nitric oxide (NO) and malondialdehyde (MDA), while suppressing the activity of antioxidants such as superoxide dismutase (SOD) and glutathione (GSH)