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Dynamic regulation of airway surface liquid pH by TMEM16A and SLC26A4 in cystic fibrosis nasal epithelia with rare mutations

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Quantitative real-time PCR RNA was purified by using ReliaPrep RNA (Promega, WI, USA) and reverse transcription was performed using 1.5 g total RNA as template with the ImProm-II Reverse Transcription System (Promega, WI, USA)