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8.8.1.4 Toxic effects of 4-DMAP on isolated rat kidney tubules Various toxic effects were observed when 4-DMAP was added to suspensions of isolated rat kidney tubules, apparently as the result of the irreversible binding of 4-DMAP to SH-containing compounds, e.g., multiple enzyme inhibition, inhibition of gluconeogenesis, decrease in glutathione, and membrane damage (Snizicz et al., 1979)